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Live-cell imaging probes developed by Spirochrome are cell-permeable compounds which stain microtubules (SiR-Tubulin), F-actin (SiR-Actin), Lysosomes (SiR-Lysosome) and chromosomal DNA (SiR-DNA) in living cells. These probes were recently introduced in a landmark papers published in Nature Methods and Nature recently featured on the cover of the Journal of Biological Chemistry. Cytoskeleton also provides Spirochrome's premier mechanosensor probes (Flippers) which can detect membrane tension changes through their fluorescence lifetime changes. You can read about the development of Flippers Nature Chemistry.
Originally developed as the MemBright™ probes, MemGlow™ probes provide high photostability, exhibit excellent fluorescent quantum yields, and some of them are super-resolution compatible. Structurally, MemGlow™ probes are composed of cyanine or BODIPY dyes bearing zwitterionic amphiphilic anchors for enhanced retention in the plasma membrane. The fluorogenic nature of MemGlow™ probes translates to minimal fluorescent emission until their contact with the plasma membrane elicits integration into the lipid bilayer. This characteristic creates minimal background, bright plasma membrane localization, and a no-wash-required application. MemGlow™ probes are highly efficient and allow scientists to visualize cellular nanostructures with nanomolar probe concentrations. For an in-depth look at MemGlow™ probes (part of the MemBright™ family) view the groundbreaking publication.
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